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Bacterial growth experiment lab report

bacterial growth experiment lab report See full list on learning-center. Bacterial Growth. Download file to see previous pages The main aim of the prevailing lab experiment is to insert the genes in order to make E. The extra water helps promote visible, well-developed bacterial growth. coli strain used in these experiments has been rendered non-pathogenic, it is important to teach the students good sterile technique and safe disposal of bacteria. glencoe. coli has been used widely in disinfectant test as a pathogen indicator. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 2. To investigate the type bacteria existing in different locations, we took a culture from the inside of a mouth and the surface of a tabletop and let the bacteria grow in agar plates for one week. 15 page lab with Introduction, Protocol (Overview and student handout for Population Dynamics—Bacterial Growth Curves Provide Data to Calculate Growth Rates and Carrying Capacity (Microsoft Word 2007 (. 2014). The bacteria at the end of our experiment could not only glow green under a UV light, but it was also able to resist a specific antibiotic (ampicillin). homesciencetools. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. Procedure: 1. coli samples and DNA for the genetic expression of fluorescence and ampicillin resistance. 5mm from its original place. Micah Schenck. Compare and contrast animal, plant, and bacteria cells by looking at images of cells and completing a lab report. A. et al, 2006). Michael J. Nothing grew in the broths in the course of Pasteur's experiment. First, the bacteria mix inside the Unknown 109 tube was streaked across a nutrient agar plate using the quadrant streak technique. SECOND PERIOD Material: 1. 2. L. Bacterial Growth Lab Report 1360 Words | 6 Pages. 5-7. This requires knowledge of optimal temperature for growth, optimal oxygen requirements, and optimal nutritional needs. P. Bacteria was taken from the MSA plate and inoculated onto a Nutrient Agar plate to grow an isolated colony. Take these petri dishes and turn upside down. Start studying Lab 12: Effect of Osmotic Pressure on Bacterial Growth. Be sure you clearly describe how you measured the 7th LAB “Grow Bacteria” LAB: Growing Bacteria Name _____ period ___ Date _____ Introduction Bacteria are tiny micro-organisms that can be found almost anywhere. BIO: 203. Based Our results showed that an increase in salinity by 25 percent led to the growth of SEM to 1. Abstract This experiment is about bacterial growth. ) 4. 3/7-9. Bio 3302 - Final Lab Report 5/14/18 1 Introduction: The identification of bacteria is important in order for us to differentiate one microorganism from another and classify them based on their morphology, ecology, genetics, and most importantly by species. The tube in 25oC: The amount of growth was slight, white in color, and had Echinulate form of growth. Perform a commonly used method of isolating bacteria in pure culture - the streak plate method. With your group, design an experiment to test for the presence of bacteria on surfaces. LB/Ara. Knowing bacterial species is especially useful Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction. In this experiment, you will perform a viable count aseptically in order to determine how many live bacteria (cfu) are in a colony that you scrape off of an agar plate Nutrient agar is enriched to allow growth of a wide variety of bacterial cultures, thus it was expected that both of the cultures used would exhibit growth on the nutrient agar. 2. coli cultures were incubated to increase the growth rate of the bacteria on our control petri dish. Genetic transformation is an active uptake of free DNA by a bacterial cell and the incorporation of the genetic information. Carefully streak the cotton swab onto the gelled medium (agar). 3/21-23 . Materials: Nutrient agar plates from Experiment 1 The results collected after the six day period of observation revealed that factors which influenced the rate of bacterial growth were temperature, pH level, moisture and the amount of food sources available. edu exp1 microbial growth 1. Lab #3: Differential & Selective Bacterial Growth Media Lab Exercise INSTRUCTIONS Lab #3: Differential & Selective Bacterial Growth Media Lab Report Template Biology Lab Report Sample, Cont’d Materials and Methods Use the information you recorded in your lab notebook as a guide to write this section in paragraph format. Coli) as the bacteria or the host organism, because E. The average of the data was calculated and presented in Table 1, showing color changes for each temperature. The paper discs were placed on the inoculated plates. intermedius grew best at 37°C. Bacteria live in communities • Bacteria are usually found in large multi-species communities • In their natural environment they coexist with other bacteria, fungi, protozoans and viruses • Specific bacteria must be isolated from the community in order to study their properties • For instance, a specific species that causes disease or The Lab Report Template is what the students use to develop the lab report of this project that will be turned in. Either then or next lab period, count the number of colonies on each plate, calculate an average and record results. The reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be seen through the microscope. Include purpose of lab experiment> <brief summary of topic investigating and case studies > <state major finding> Procedure < Include information that the reader would need to repeat your experimental procedure. Coli donor cells were each plated onto MacConkey (MAC) agar plates loop and placed into a sterile test tube regulation by cell size control of initiation, amp; Tatum, E. 2. Discussion . In this laboratory exercise, you will work in groups and follow the normal growth curve of an unknown bacterial species to determine its generation time at different temperature and aeration conditions. Through experiments performed in the lab, students will learn about the biology of bacteria, methods with which to work with bacteria, how morphological and physiological traits can be used to identify bacteria, and how to control bacterial growth. way of gene variability in bacteria. Materials & Methods: I sampled the bacteria by using swab, the gloves and the Petri Dish with Agar. 20 14. Growing Bacteria in a Lab: Experiments & Conditions. Bacteria Transformation in Biotechnology Essay 1673 Words | 7 Pages. Chemical agents have been used for centuries to control the growth of unwanted bacteria. By knowing what diseases are caused by different types of bacteria and their modes of action, healthcare workers can treat patients with the proper medication. S. The growth hails a research conducted by Callaway, & Kao (2014), who found out that the osmotic stress brought about by dense populations or high concentrations of osmolyte can significantly impact on the productivity and growth of microbes. How do you think the bacteria grew in such a short time? (Bacteria reproduce using binary fission. results in rapid absorbance increase in the bacterial growth curves. Education Center - K-12 Lessons and Laboratories - Classroom Activities in Plant Biotechnology: Activity 4 - Transformation of E. fungi. The control group after incubating at 25 degree Celsius, showed the growth of red colonies. Bacteria(in(Yogurt(Lab(! Introduction(Some!types!of!bacteria!can!ferment!milk,!producing!lactic!acid!in!the!process. All in all, the lab was fun and a success in transforming the bacteria, e-coli, into one that is antibiotic resistant and into one that can glow. It is often very difficult to replicate bacterial growth conditions in the lab. This lab report will focus on controlling the growth of bacterial growth. Set up an experiment and interpret your results. The lab is split into two parts and takes five days for germs to become visible. The two forms of bacterial growth seen are with both the +pGLO plasmids because its genes connect with the LB broth, ampicillin, and arabinose. You will be given sterile cotton swabs and petri dishes containing nutrient agar on which bacteria can grow. Microscopes can then be used to identify specific bacteria. 3. Describe components of a lab report (SLO 2) Lab #1: Introduction to Science Experiment 1: Design an Experiment Design an experiment to test selected variables that may (or may not) affect yeast fermentation. E. Short report for Courtney Zahn. Nature. To demonstrate the presence of molds and the conditions required for their growth, the popular bread experiment is done. The two forms of bacterial growth seen are with both the +pGLO plasmids because its genes connect with the LB broth, ampicillin, and arabinose. Describe components of a lab report (SLO 2) Lab #1: Introduction to Science Experiment 1: Design an Experiment Design an experiment to test selected variables that may (or may not) affect yeast fermentation. Other parts of the experiment I learned new facts relating to bacterial growth. Coli bacteria will glow, and grow the most under +pGLO with LB/Ara/Amp. growth. Phenolphthalein is used In the past several weeks, we have done a bacteria growth lab in the science class. 1% of all bacteria have been successfully cultured. The other bacteria which was microscopically viewed as gram positive was missing in the incubator, another tube of sample was derived from the lab professor. The growth of all strains evaluated was inhibited with a 0. If autoclaves or pressure cookers are not available, an alternative is to bleach the plates. laboratory report biochemistry and microbiology (bft 1014) members and matric no. Gram Stain Report Introduction The purpose of this experiment is to determine the shape and Gram stain of the bacteria under a microscope. 7 Regulatory issues [in food preparation]. (1)donny liang khai chiun b11a056 (2)khor yee heng b11a135 (3)looi kah shien b11a165 (4)yip hon lean b11a438 (5) yong woon sing b11a442 (6) lau su hui b11a565 group sbp 1 date of experiment 8th may 2012 title of experiment experiment 7: staining techniques in microbiology experiment 8 2016 Microbiology Laboratory Manual – Page 22 antibiotics are placed on the agar surface. 1. Practice writing a lab report 2 hours LM #1 Introduction to Microbiology & Classification Overview of Microbiology 1. The largest average in movement was in Streptomycin, which moved 8. STUDY. Concentrations of bacteria cells in samples are usually too great to be counted directly. HYPOTHESIS: I predict that as the time the bacteria is allowed to grow gets longer, the amount of bacteria will increase until all of its resources are depleted. ” 3 State your hypothesis at the end of the Purpose section. 5 5 5 5 2. This process is used frequently to study the growth of organism. Also, our experiment supports the notion that that the GFP gene is activated by the sugar arabinose, which is what lets jellyfish and our E coli fluoresce. Make sure media required for the experiment are experiment with many nutrients and growth conditions to culture the newly isolated bacteria in the lab. Incubate both tubes on ice. Parameters such as pH, temperature, oxygen concentration, salt Set up a serial dilution of a bacterial culture and describe why they are used. With this in mind, it is important for laboratory BIOC1010: Introduction to Microbiology Laboratory report: experiment 3 Colony growth and analysis of two bacterial strains Introduction The use of solid media for the culturing of microorganisms allows the isolation of clonal lines in which all the cells in a colony are derived from a single progenitor cell. Bacterial Transformation Lab Report. This will be an actual lab report you will write. 4. The purpose of this experiment is show how numerous special media are available that can help in isolating bacterial types, differentiation among closely related groups of bacteria, enumeration of bacteria in sanitary microbiology, assay of naturally occurring substances such as antibiotics, vitamins, and products of industrial fermentation, and last but not least characterization and identification of bacteria by their abilities to produce chemical changes in different media. These agents could prove useful in the Objectives: After completion of this laboratory experiment, the student will be able to: 1. A digital notebook (Google or Powerpoint) is included in this resource. 4. Growth rates were obtained in laboratory medium by using a binary dilutions method in which 15 optical density curves were generated to determine one mu value. amrita. Once enough growth has been established, observe and identify the BHI slants and broth tubes for growth. com Synthetic Biology One is a free, open online course in synthetic biology beginning at the undergraduate level. The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references. Analysis/ Interpretations: The zone of inhibition represents the average length the bacteria moved in the lab. This experiment is based on the transformation mechanism of bacteria and gene regulation. In this experiment, agar plates were contaminated (i. LAB Report #3 Introduction: In this lab we have focus on Isolation of bacteria from environment. This report does NOT contain the required bibliography or internal references. Understand and perform direct measurement of bacterial growth through serial dilutions and standard plate counts. In this study, a disinfectant experiment was conducted using 70% laboratory ethanol, 10% commercial bleach and 50% commercial antibacterial hand soap against pure E. All bacteria will have their DNA in the form of a single circular chromosome. Two weeks later, they complete the biology portion of the lab when they collect their specimens and determine which coated surfaces controlled the bacterial growth best. The aim of this experiment is to follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the stationary) during one day culture. Thermophiles are heat-loving bacteria. 2. Any laboratory procedure you follow without a hypothesis is really not an experiment. You should now understand the different growth phases of bacterial cultures, how to perform an experiment to obtain a growth curve using time point collection and serial dilution plating, and how growth analysis can be applied to research and industrial purposes. Lab Report 3 Bio 1316L Dr. figure: pH vs growth rate. This bacteria growth science experiment is part of my Summer STEM Lab series of posts aimed at school age kids. After the experiment, our hypothesis was proven correct. The agar plat store inside the incubator (35 o C overnight). 12/6/12. WEEK 6. Remove the lid from the agar plate but hold it closely over the top of the plate to use as a shield to prevent airborne contamination of the plate. Lab 6. If you want to do a science fair project about germs, you have to add a variable, or something that changes in the experiment. Count the number of bacterial cells in at least 10 small squares. How Are Hypotheses Written? Chocolate may cause pimples. BACTERIA ON FOOD LAB DOCUMENTS The Lab Project Instructions provide a thorough explanation of the lab topic, as well as detailed directions on how to complete the lab exercise. The temperature was in the range from Draw what you observed: . Utilize the Lab Report #4 Form as the starting point of the lab report that you will create. Control:(-DNA/+AMP)No colonies were formed, showed no growth with the presence of AMP. coli using green fluorescent protein Information for TeachersSafety Instructions Although the E. Results. The edge of this zone correlates with the antibiotic concentration that inhibits the growth of the bacteria. Unnumbered list of materials needed in the experiment; should not include the obvious (pencils, graph paper, desk, et cetera). Scientists grow bacteria cultures when they want to study bacteria in a lab. 333333 2 Microbiology lab tutorial on Mannitol Salt Agar (MSA) specialized bacterial growth medium. coli has been used widely in disinfectant test as a pathogen indicator. Molds, on the other hand, are more tolerant of hypertonicity. As we expected, the bacteria did grow better in the cold temperature; however, when the temperature is increased, we found that the bacteria did not grow as well. PDF of a nine page lab packet (includes safety agreement) for conducting an experiment on germs/ bacteria growth in the classroom and on the students' hands. Plant growth may be affected by the color of the light. Environmental regulation of microbial growth/ Partial Lab 4 report due 3/14 (Results and Discussion) 3/14-16. bacterial growth curve A curve on a graph that shows the changes in size of a bacterial population over time in a culture. This will be a vital task to take with me into my profession for many reasons. The procedures from the laboratory manual by McDonald were used to accomplish the tests needed to find the two unknown bacteria (3). Laboratory report 4: effect of environmental conditions on bacterial growth Outline: Leboffe Ex. Lab 6A – Bacterial Transformation & Ampicillin Resistance Introduction: Bacterial transformation occurs when a bacterial cell takes up foreign DNA and incorporates it into its own DNA. S. A lab report differs from a paper in that it has defined sections. Coli using GFP. It is often very difficult to replicate bacterial growth conditions in the lab. BACTERIAL GROWTH and CONTROLLING BACTERIAL GROWTH lab report using virtual unknown software. Calculate the cfu/ml from colonies counted on an agar plate, given the dilution factor and volume correction factor. Your instructions are 1) to determine the exponential growth rate of this bacteria, and 2) to determine how long the medium continues to support exponential growth. Use selective media to isolate an organism from a mixture of organisms. Example, maybe more bacteria growth or more transformations so the changes become really clear. disks with various chemicals then place the disks on a confluent lawn of bacteria and look for a zone of inhibition (clear. The results show that the bacterial growth curve on PET exhibits the distinct lag and log phases, but the generation time is more than twice longer than in bulk medium. In this study, a disinfectant experiment was conducted using 70% laboratory ethanol, 10% commercial bleach and 50% commercial antibacterial hand soap against pure E. 1% concentration of acetic acid in the vinegar. To do this, start at the top and work down in a zigzag motion. Conclusion: The purpose of this lab was to see how effective an antibiotic is to stop the growth of bacteria. The size of the zone of inhibition is proportional to the growth inhibiting properties of the. The bacteria we work with are also very easy to culture in the lab. I am doing this experiment to find out : where can I find lots of bacteria? I predict I will find lots of bacteria on the keyboard in Jordan's room. For bacterial strains, E. MCB 3020L Lab Experiment 5a & 5b Winogradsky Column (5a) and Enrichment Culture (5b) A long term (5a) and Three Session Experiment 5a Winogradsky Column. a. Purpose: 1. Unknown Number 116. LAB REPORT Biochemical identification of unknown bacteria Unknown ID 001 Nerjes Alsalman 3/18/09 Biochemical identification of unknown bacteria RESULTS: Morphological characteristics: Nutrient Agar Slant: The tube in 37oC: The amount of growth was slight, white in color, and had effuse form of growth. Chemicals The Penicillin had colonies of bacteria re- growing, so this would make it the least affective of keeping the bacteria gone permanently. Then a 1%, 5%, and a 10% concentrated solution of the acid and the base each was created, and applied to 6 paper discs each. You have been given a mystery bacteria and growth medium by your instructor. Agar is a gelatinous material extracted from seaweed that forms a moist surface favorable for bacteria reproduction. For bacterial strains, E. This simple experiment not only demonstrates the importance of hand washing but also highlights the necessity of using aseptic techniques when studying bacteria in a lab. You need a reliable technique lab report your results of and biochemistry is bacillus are also requires incubation for bacterial isolates to this experiment. Amy Kaiser. This experiment is based on the transformation mechanism of bacteria and gene regulation. In this lab you will be innoculating plates and observing bacterial growth. After 30 minutes submerge both tubes 3/4 of the way into the water of a 42 °C water bath for 30 seconds. We welcome scientists, artists, journalists, p 4. There were 24 tubes of sterile, semi-solid media that served as a source of water and minerals. After an overnight incubation, the bacterial growth around each disc is observed. A bacteria will grow to twice its size and then split into two “daughter” bacteria with the same genetic code. As bacteria grow and reproduce in milk, the pH level of milk drops. However there are certain bacteria that grow best at acidic or basic pH. The procedure employed in the experiments reported was as follows: TABLE1 Theeffect oftime, temperature, andgrowthontheresults obtainedbytheplatemethod onmilk oflow bacterial content AGAR HELDIN PLATEDIM- POURED DILUTION MEDIATELY AFTER few bacterial strains. Microbiology lab report - exercise #6. As the lysis of dead bacteria is slow, the absorbance of the total bacteria mass won t decline dramatically in 24r 32 hours. Are you surprised by the amount of growth in your sample? 3. of nitrogen and their effects. , 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls, Clin. 4. If the substance is inhibitory, a clear zone of inhibition will surround the paper disc. After inoculating complex TSA and selective SA media with a sample form oral cavity, the bacterial growth occurred in about seventy-two hours at 37 0 C on the TSA plate but not on a SA plate At the end of experiment, plot a graph of time in minutes on X axis versus optical density at 600nm on Y axis to obtain a growth curve of bacteria. For your lab report you will be using the ampR plasmid from the virtual lab. We work with a very limited number of bacteria in this course. A) We started the inoculation process by labeling our petri dish on the bottom. It is estimated that only 0. We will demonstrate a bacterial growth curve using a closed system. Easy it is aseptic lab report your findings in obtaining growth of and action potentials, some large colony and biochemistry. Background. The Winogradsky column is made to provide enrichment culture for anaerobic photosynthetic bacteria and sulfur cycle bacteria from a natural assemblage of At the conclusion of the experiment, all plates should be disinfected for safe disposal. Musliu Abdulkadir and Salawudeen Waliyu, Screening and Isolation of the Soil Bacteria for Ability to Produce Antibiotics, European Journal of Applied Sciences. Enumeration of microorganisms is especially important in medical microbiology, food and diary microbiology, and environmental microbiology. Bacteria Transformation in Biotechnology Essay 1673 Words | 7 Pages. (4, 5, and 6) Results. The differential ingredient is lactose. Hypothesis temperature will have an effect on the growth of bacteria in milk. Drosophila fly lab report laboratory reared versus wild captured wf fig4 2203. coli strain and mixed culture collected from aerobic batch reactor. Bacterial Growth: Binary Fission Microbial Growth Growth of Bacterial Cultures Logarithmic Representation of Bacterial Growth: We can express the number of cells in a bacterial generation as 2n, where n is the number of doublings that have occurred. Experiment : (+DNA/+AMP)On plate 300-500 colonies have grown and appear to be white without under the UV light. Gene recombination in Escherichia coli. Theory to be discussed in the next lab Refer to Table 3 Instructor will demonstrate and explain the experiment . At the end of the growth period, tubes are inverted 3 times and pH affects the ionic properties of bacterial cell so it affects the growth of bacteria. Aquire 1-2 agar plates. experiment is a repeat of an earlier experiment, you do not have to write down each step, but can refer to Microbiology Laboratory Manual 5 Naveena Varghese & Joy P. caliente grew best at 60°C, but not below 37°C. Antibiotic resistance in bacteria is a serious global public health threat. Experiment 2 (Lab Periods 2 and 3) Determining the Number of Bacteria in a Colony Bacteria are found throughout the biosphere, inhabiting places that eukaryotes find uninhabitable, sharing all the places where eukaryotes live, and living in and on the bodies of eukaryotes. This transformation usually occurs within plasmids, which are small circular DNA molecules separate from its chromosome. The gram positive bacteria was subjected to the following test; First the gram (+) bacteria was tested on catalase test. Lab Readings linked on the Differential & Selective Bacterial Growth Media Laboratory Main Page of the Virtual Microbiology Classroom Purpose: The purpose of this laboratory is to provide opportunities for student (1) practice the techniques of pouring and inoculating media (2) interpret growth of bacterial culture on media and (3) apply the characteristics and uses of a small selection of media. uv controls bacterial growth by causing irreparable damage to the bacterial DNA so the experiment would be less Note: Find more science experiments on my Science Activities for Kids page! We started by talking about how bacteria are single-celled organisms that are neither animals nor plants. In the medical field bacteria and infections of different kinds are the core of the practice. Title: Antibacterial properties of Various Cleaning Agents. Practice writing a lab report 2 hours LM #1 Introduction to Microbiology & Classification Overview of Microbiology 1. Pathogen bacteria are sometimes referred to as germs. Perform essential aseptic techniques. It is believed that the milk placed in a warm place will have more bacteria growth than the milk placed in a refrigerator in an Erlenmeyer flask. Place the flask in a shaking 37qC incubator to start the bacterial growth. coli strain and mixed culture collected from aerobic batch reactor. Abbott. Positive – A diffuse cloud of growth away from the inoculation line is evident. If the test isolate is susceptible to a particular antibiotic, a clear area of “no growth” will be observed around that particular disk. The optimal temperature for growth may vary among species. 666667 3. Equipment and materials for this laboratory are available from the Carolina Biological Supply Company (see Appendix 1). Designing an apparatus to collect bacteria; Developing a research design to test an idea bacteria in a given sample, as well as having to compare the amount of bacterial growth under various conditions. Once competent, the cells are ready to accept DNA that is introduced from another source. Know the different phases of a standard growth curve. Short report for Lab 3 due 3/7 . Biochemical tests. Feel free to swab any surface on your person, on your bag, in the laboratory, or outside of the classroom. The zone of clearing surrounding Bacillus megaterium and Bacillus subtilis indicate that both were able to hydrolyze starch. Case study involves a study that was to see if changing the diet of his animal would affect her skin. Simultaneously, TSIA test was performed to determine the fermentation of carbohydrase, gas and hydrogen sulfide. 1. Lab Report Of The Experiment Of Conjugation Of E. Sample Lab 1 Report - 2 Lab Report 1 (Sample) Serial Dilution of a Bacterial Culture Introduction Genetic analysis often requires the measurement of the frequency of events that are not likely to occur except in a large sample. Observati on:There is no bacteria growth on the culture medium. This indicates the succession of bacteria in the liquid. of nitrogen and their effects. Janda and Sharon L. At this time, the cells are said to be competent. e. 6. This static action of osmotic pressure thus prevents bacterial decomposition of the food. Question/Purpose: The purpose of this experiment is to determine if Lysol disinfecting wipes, hand soap/detergent, hand sanitizer, and water are good antibacterial agents by viewing agar plates which have been inoculated with bacteria from a cell phone, and then comparing the results. coli resistant to ampicillin and to glow. I use this lab in my Biology I classes, but it can easily be used for a middle school science class, or a more advanced Biology class. coli resistant to the corresponding ampicillin. 6. The report presents the results of a microbiological experiment to confirm the hypothesis that as leftover food sits out over time, the number of bacteria in the food will increase. Bacteria Transformation in Biotechnology Essay 1673 Words | 7 Pages. And, the bacterial isolate’s susceptibility and resistance to a variety of antibiotics were both tested following the protocol for the Lab 9. Gelatin hydrolysis test is used to detect the ability of an organism to produce gelatinase (proteolytic enzyme) that liquefy gelatin. Bacterial adhesion The ability of bacteria to adhere to surfaces is tested by growing bacteria in test tubes containing a coverslip. Bacterial growth also requires tightly controlled environmental conditions. The other two plates showed no growth. Transfer See full list on vlab. 3. Chapter 5 Bacterial Growth Micro-organisms such as bacteria can be grown in the laboratory if essential components required for their survival are present. In this lab students will observe the effect that different antiseptics and disinfectants have upon the growth of bacteria. The study was undertaken to indicate the risk of bacterial contamination and the importance of proper storage technique. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. 2. a variety of antiseptics and disinfectants on bacterial growth. area) surrounding the disk. If your instructor gives you an outline for how to write a lab report, use that. 2-12 Chemical Germicides 8 Lecture: Reading: Epidemiology of food-borne Montvilleillnesses : Handout: Introduction to epidemiology Ch. , we took extracted DNA and inserted them into E. bio-rad. Microbes in Action: A Laboratory Manual of Microbiology. Transferring an isolated bacterial colony from one plate to another or to a different type of growth surface is an important technique in microbiology. Microbiology (Lab Report) Closed system growth curve Closed System Growth Curve Lab Report PURPOSE Bacteria grown in a closed system show a specific growth pattern called the growth curve which consists of four phases. The canning of jams or preserves with a high sugar concentration inhibits bacterial growth through hypertonicity. These media contain all the elements that most bacteria need for growth and are non-selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory-culture collections. 3. Bacterial growth may be affected by An experiment can be preformed to monitor the rate of bacterial growth in milk by testing the acidity of the milk liquid. The microorganisms are called the inoculum. coli (EHEC) O157:H7 were examined. Within the bacteria cell DNA is replicated for cell division (binary fission), DNA is transcribed into m RNA that will travel to ribosomes where it gets translated into proteins. Teichoic acids also consists of lipotechoic acids which have a lipid component that attaches to the cell membrane (Slonczewski & Foster, 2015). 3. experiment with many nutrients and growth conditions to culture the newly isolated bacteria in the lab. According to the Centers for Disease Control and Prevention (CDC), 2 million people in the U. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. Also, it could be used to see what other genes can be turned on or off. Iodine has been added to this starch agar plate. 4. Colony counter Procedure: 1. Huge List of Example of Biology Lab Report, Experiments Topics, Models Ideas for Kids and also for Middle school, Elementary School for class 5th Grade,6th,7th,8th,9th 10th,11th, 12th Grade and High School ,CBSE, ISC Class 12 and MSC and College Students. This experiment is based on the transformation mechanism of bacteria and gene regulation. Because disinfectants and antiseptics often work slowly on some viruses - such as the hepatitis viruses, bacteria with an acid-fast cell wall such as Mycobacterium tuberculosis, and especially bacterial endospores, produced by the genus Bacillus and the genus Clostridium, they are usually unreliable for sterilization - the destruction of all life forms. This is because higher temperature can let the organism’s proteins and enzymes, denaturation or coagulation. Yes, it is gross and cool and fascinating, but it doesn’t meet the requirements of a science fair project. The agar is appearing to be yellowish in colour and there is no white spot appears on the surface of the agar. We hoped to find a solution that would both inhibit bacteria growth and that is also safe and sustainable, suitable for common household use. Bacteria on general or selective media and assessing growth in response to. Incubate for 18 hours at 35 degree Celsius or until such time that growth is noticeable. Samples are removed at intervals and the number of viable bacteria is counted. In each experiment, the chemical agents had a similar effect regardless of the specific bacteria. It is just an exercise or demonstration of what is already known. 158, 558. This lab may take several days, keep all data and observations in a separate notebook to be compiled and organized into a final lab report. This will not be in question-and-answer format, like the pervious lab report. Abstract: See full list on milnepublishing. Ubiquity of Bacteria - pg. - Make sure that all bacteria is contained and that hands are always washed after working with samples. This means that Streptomycin was the most powerful antibiotic used in this lab, so the bacteria must have lied on it. It proves that it contains normal (red) strains of S. McCracken agar (MAKE) is selective for certain types of bacteria and is known to inhibit growth of Gram-positive microorganisms. There can be 10 to 200 copies of … Continue reading "Sample 6a Transformation Lab" Lab Report on Plant Growth At the beginning of the semester, your instructors set up an experiment to determine whether two hormones, auxin and cytokinin, affect the growth of lettuce seedlings. Clostridium butyricum Escherichia coli Pseudomonas aeruginosa 3. (1946). Also, healthcare workers can help prevent the spread of disease by knowing how different types of bacteria are passed. Fermentation of this sugar results in an acidic pH and causes the pH indicator, neutral red, to turn a bright pinky-red color. Students will be able to graph results. The bacteria are grown in a sterile petri dish containing agar. Introduction. We used anaerobic conditions with light and a medium containing a non-fermentable carbon source, malate for the enrichment. Ampicillin is an antibiotic that is known to treat bacterial infections, in this experiment ampicillin’s role is to kill all bacteria that did not undergo a transformation. The second part of the thesis project was to study the kinetics of fading of phenolphthalein in sodium hydroxide solution in order to provide a basis for experiments illustrating this in laboratory environment. 4. edu This report analyzes an experiment conducted in order to transform E. Step 1:Do the initial inoculation then FLAME the loop, let cool about 15 seconds. The purpose of this lab was to develop an understanding and appreciate the results of transformation as well as focusing on the effects of ampicillin on the pVIB plasmid. M/Tu, March 1/2, 2021. Antibiotics. The null hypothesis of this experiment is that there will be no difference in bacterial growth between the plates. The bacteria growth will enter decline phase within 24 hours of culture. A hypothesis is an educated guess about the outcome that you expect to reach by conducting the experiment. This experiment was therefore on study of various bacteria types through isolation, culture and sensitivity against antibiotics like tetracycline. Sterile&technique&is&the&technique&used&to&prevent&contamination&of&sterile& material being used& during a lab (Sterile Lab Exercise 13: Growth Curve OBJECTIVES 1. In the Transformation Lab designed by the Carolina Biological Supply Co. Salt in soil may affect plant growth. There will not be formal lab discussions on this day. Easy it is aseptic lab report your findings in obtaining growth of and action potentials, some large colony and biochemistry. The same effect is obtained by salt-curing meats or placing foods in a salt brine. The lab is 100% digital and no lab equipment is required. Bacteria Growth in Milk Purpose 100 The purpose of this lab is to determine if temperature has an effect on the growth of bacteria in milk. The following is report that repeats the two classic experiments that were used to disprove Spontaneous Generation--that life originates from non-living substances. Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this lab experiment two different types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. 333333 2. To arrive at the best bacteria enumeration lab report, you may need the assistance of expert lab report writers. (4, 5) Lab Report 4 is due in LON-CAPA by 11:59pm, 40 points Exercise 4 Student Rubric Data to use for Experiment 4 Lab Report ppt. Genetic transformation is the active uptake of the free DNA by the existing bacteria cell coupled with the incorporation of the underlying genetic information. To provide good aeration for bacterial growth, the flask volume should be at least four times the total culture volume. , colonies grew) when exposed to human breath only briefly. Competence can be induced in E. Some bacteria can reproduce once every 20 minutes. 166667 5 4. 37=organisms with warm-blooded cells 22-25=organisms that grow on glaciers 3. geneseo. The bacteria are cultured in sterile nutrient medium and incubated at the optimum temperature for growth. I also learned that colder temperatures do fight off bacterial growth. The diffusion of the antimicrobial agent into the seeded culture media results in a gradient of the antimicrobial. 72 mm from 1. I hypothesized that if Penicillin is applied to the bacteria, then the zone of inhibition will be Name:& & &Block:& Discussion&Questions:& & 1. Introduction. , 2012, 4, 211-215. Bacteria strains tested in this experiment include both gram positive and gram negative. Students could answer the questions at the end, or they could write a lab report as the final product. Experiment 9: Growth curve of Serratia marcescens Abstract Bacteria grows by binary fission. Plate cover should not be removed until instructed to do so. The lux operon is an operon that contains a gene for luciferase and a portion of the gene Teacher Visualizes Bacterial Spread With Bread Experiment Sponsors Schools can hang up posters, make announcements and remind students directly to wash their hands frequently during flu season, but ultimately, many students from kindergarten through high school simply don’t have the perspective to recognize the consequences of spreading germs. In this experiment plasmids, are inserted into a host E. Antibiotics, which are used to treat bacterial infections, are also tested against their sensitivities to various bacteria in the lab using a sensitivity chart in various bacteria cultures. Biotechnology Explorer™ pGLO™ Bacterial Transformation Kit Catalog #166-0003EDU explorer. Sterilization, cleanliness, and being attentive to your experiment is pertinent. Mohamad Zan. Experiment II: Controlling Microbial Growth. UNKNOWN LAB REPORT. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. 2. Firstly let’s begin to discuss the Gram Staining before getting to the Aseptic Technique. The impregnated disks are commercially available and greatly facilitate testing. The best way to dispose of bacterial cultures is to pressure-sterilize (autoclave) them in a heat-stable biohazard bag. et al, 2006). The two forms of bacterial growth seen are with both the +pGLO plasmids because its genes connect with the LB broth, ampicillin, and arabinose. Microbial Growth Phases of Growth Bacterial Growth Curve : When bacteria are inoculated into a Factors Affecting Growth of Bacteria The growth of microorganisms in the body, in nature, or in the laboratory is greatly influenced by temperature pH, moisture content, available nutrients, and the characteristics of other organisms present. chemical. Using the correct strategies to control bacteria is very important. Engineering & scientific process skills students will practice while conducting this lab. Methods This portion of the lab report will discuss everything that you did: from collecting the bacteria and plating the mouthwash to reading the results of the growth. Temperature did limit the growth of the three bacterial species, but only V. The final stage of bacterial growth by Bacillus Microorganism Lab Report A sample of bacteria was aseptically removed from D4 and inoculated in to HEA, MAC, MSA and starch agars Medias. Section 2: Microbial Growth; Ex. The test consists of spreading a bacterial inoculum on a nutrient agar plate, and placing paper discs that have been soaked with various concentrations of antiseptics or disinfectants on the plate. way of gene variability in bacteria. Differences encountered in a Real Laboratory: In an actual laboratory setting, there are certain important steps that are not necessarily applicable in a virtual lab: Always wear lab coat and gloves when you are in the lab. When you enter the lab, switch on the exhaust fan and Laminar Air Flow. Variables Independent Dependent Constant Experiment Test Group Control Group 3. bacterial susceptibility to the antimicrobial present in the disk. The TSIA test was followed by SIM test. Bleach proved the most effective, household disinfectant a close second, with anti-bacterial soap proving the least effective in each experiment. When the concentration of the antimicrobial becomes so diluted that it can no longer inhibit the growth of the test bacterium, the zone of inhibition is demarcated. Lab 5. S. A clearing around the bacterial growth indicates that the organism has hydrolyzed starch. Factors Affecting Growth of Bacteria The growth of microorganisms in the body, in nature, or in the laboratory is greatly influenced by temperature pH, moisture content, available nutrients, and the characteristics of other organisms present. Disproving Abiogenesis: The bacteriostatic and bactericidal actions of vinegar on food-borne pathogenic bacteria including enterohemorrhagic E. Based The two forms of bacterial growth seen are with both the +pGLO plasmids because its genes connect with the LB broth, ampicillin, and arabinose. In this lab we are investigating Prokaryotes (bacteria) using two techniques, Aseptic and Gram Staining. The procedures that I included are for a face-to-face lab that uses a different plasmid, but the steps are the same. We have designed the laboratory exercises in this book around a few major concepts-proper use of aseptic techniques, bacterial staining and microscopy, bacterial metabolism, and control of microbial growth. . View Lab Report - Lab Report 1 (BIO 280) Ubiquity of Microorganisms from BIO 280 at JMU. Coli bacteria. In this paper I will discuss the processes of how I came to find my two unknown bacteria. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. 4/29/2014. Had the plates been left open during inoculation, bacteria present in the air and other surfaces could have contaminated the culture producing incorrect experimental data. The unknown gram-negative bacteria is Enterobacter aerogenes. These results are significant since plating is one of the most basic and common of experimental methods to detect and count bacteria in environmental samples. In this experiment, you will soak filter paper. Lab 3. Experiment 9: Growth curve of Serratia marcescens Abstract Bacteria grows by binary fission. Laboratory 5: Growth Curve This experiment is found in LON-CAPA. Turbidity, which is a measure of the growth of microorganisms, is determined via an instrument called a spectrophotometer. Here's a format for a lab report you can This is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown. develop antibiotic-resistant infections each year, and at least 23,000 people die from those infections. This experiment proved that in order for recombination to occur, the two strains must come in contact with one another (Lederberg, Tatum, 1946). Indeed, the bacteria cells grown in the culture with 25% nutrient broth produced only 25% of the yield from 100% nutrient culture. Considering the gathered facts and information from the conducted experiment, it can be said that the factors of temperature and nutrient indeed have a significant effect on the growth and yield of E. Your instructor may not be around during the tests, but you will be required to display your expertise through a lab report. This is how I learned about bacterial growth. polarensis grew best at 4°C, but not above 23°C. relationship between pH and bacterial growth is given in figure below. Makaila Stuckey March 5, 2020 Phototrophic Bacteria Lab Report Microbiology Lab 807 Purpose: The purpose of this experiment was to enrich for photoheterotrophic bacteria from sediment samples and to isolate for them as well. Their optimum growth temperature is between 45°C and 70°C and are comonly found in hot springs and in compost heaps. Huge List of Biology Lab Experiment | Biology Lab Report, Experiments Topics, Models Ideas for Kids and also for Middle school, Elementary School for class 5th Grade,6th,7th,8th,9th 10th,11th, 12th Grade and High School ,CBSE, ISC Class 12 and MSC and College Students. The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria. Unknown Lab Report. Students will be able to analyze bacterial growth before and after sanitation using UV light. A host of different types of bacteria live within your body. The Gram stain demonstrated Gram-positive cocci. Identify the amount of growth by placing a +++, ++, +, or – in your worksheet depending on the amount of growth that occurred in the tubes. The different is that one glows but it has bacteria growing over the glowing colonies and the other has an arabinose resistance that allows the glow in the colonies to be seen. Bacterial transformation is crucial for in science because it allows genetically For example, begin your Purpose statement by writing something like, “In this lab experiment, 3 different types of bacteria were separated using a nutrient agar plate. These bacteria can take up DNA only during the period at the end of logarithmic growth. Variability should be less than +/- 10%. The different is that one glows but it has bacteria growing over the glowing colonies and the other has an arabinose resistance that allows the glow in the colonies to be seen. Data representative of the results obtained in manyexperiments are presented in tables 1 and 2. Students will be able to conduct an experiment testing for bacterial levels in water. com In the laboratory, transferring a bacteria population of microorganisms to a growth medium (petri dish), is referred to as inoculation. environment which will kill the bacteria and prevent growth. coli cell. When providing experimental report writing assistance, our lab report writers recommend that students should be aware that most foods have been found to constitute nourishment for microorganism growth. Label each section with the area you indent to introduce bacteria for. 1% of all bacteria have been successfully cultured. Refer to Appendix Figure 4 to identify the types of growth found in the BHI broth tubes. 4. The zone of inhibition is measured in millimetres by using ruler and internal calliper. NO CLASS-Spring Break! 3/28-30. 2. coli with carefully controlled chemical growth conditions. bacteria control group will also give them a good indication as to how much rinsing they need to do. Temp (°C) 0 40 60 95 Time (min) 0. V. Remember to pull plates and refrigerate after 48 hours max. Sample Formal Lab Report. Bacteria Lab Report: AIM: The aim of this experiment is to find out how time affects the growth of bacteria by counting the surface area of the bacteria. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. Step 3: Use the sterile cooled loop and draw of the agar surface in the second section. 3. As you read through each exercise, perform the experiments, and interpret your results, try to always remember the “big So, your job is to figure out when the exponential part of growth is over. and growth. Created as a video assignment for the CTL7026Y - Curriculum and Teaching in Science General course of the MT program at OISE, U of T. Lab 4. This experiment is based on the transformation mechanism of bacteria and gene regulation. The chart below, displays our results after two days of bacterial growth. Antibiosis is now deeply integrated into a modern way The structure of a typical bacteria will have a plasma membrane and a cell wall. 57 mm. com MLT 415 -Lab Report (Antibiotic Sensitivity Test) M. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes, walls, furniture; in soil and dust; and on and in our own bodies (skin and mucous membranes). To learn to grow bacteria on agar in a Petri dish. Their optimum growth temperature is between 25°C and 45°C. Biochemical Test Lab Report 1371 Words | 6 Pages It aids in the differentiation of species from the genera Corynebacterium, Clostridium, Bacillus, Bacteroides, Fusobacterium and members of Enterococcus. 3. Full Lab 5 report due 3/28 . What is good about this procedure is it does not only check for bacteria’s motility, but it also helps determine other metabolic characteristics. Abstract The purpose of this lab was to insert genes that would make E. The Gram stain is a type of differential stain that allows a (micro)biologist to Date _2-20-20 Name: Lab Report: 11 Gas Requirements for the Growth of Bacteria 1 What was the purpose of this laboratory experiment? ard t1 boctania loeabeal on To Dolatbt aml tola vt their geua Draw the growth pa tterns in thioglycollate broth for each of the bacteria. Experimental Design and the Bacteria lab 1. Ubiquity of microbes: Touching a Plate. Most bacteria are mesophilic and include common soil bacteria and bacteria that live in and on the body. Experiment 2: Bacterial Transfer to a Stab Tube and an Agar Plate. The different is that one glows but it has bacteria growing over the glowing colonies and the other has an arabinose resistance that allows the glow in the colonies to be seen. intermedius grew best at 37°C, but not at 4°C or 60°C. 3. Understand and perform indirect measurement of bacterial growth through spectrophotometer readings and optical density measurements. !Yogurt! is!a!product!of!fermentation Bacteria Transformation in Biotechnology Essay 1673 Words | 7 Pages. They are so tiny that we can’t see a single bacteria with our naked eye, but we can see bacteria when it grows in clump-like groups. Some examples of this are: After performing my experiment I learned that heat does not kill off bacteria as much as I thought it would. Students will be able to describe the colony growth of bacteria from various water samples. Just growing bacteria in a Petri dish is not a science fair experiment. way of gene variability in bacteria. DESIGNING AN EXPERIMENT (Experiment Design) variables constant experimental control dependent independent 2. You could absolutely do this science experiment with preschoolers but I wanted to share some of the STEM fun I am having with my 6 and 9-year-old this summer here on the blog so I created this series. com For technical support call your local Bio-Rad office, or in the U. , call 1-800-424-6723 5. 7 blood agar plates each were inoculated with the two types of bacteria. Some instructors require a lab report to be included in a lab notebook, while others will request a separate report. marcescens. 5). The cat of growing bacteria in the lab is referred to as culture and the environment on to which the bacteria is grown is called culture medium. Lab Report Bacteria Bacteria can be found in plants and animals, in all habitats, on all surfaces and even in our bodies. Expected Result of Bacterial Growth Curve A logarithmic growth curve is obtained showing the changes in size of a bacterial population over time in the culture. Provide enough information so that the reader could repeat the experiment, but not so much that it distracts the reader from understanding the overall experiment. 332 LABORATORY 2 331-350 DNA Sci Lab 02 11/9/05 11:54 AM Page 332 You may be great with your laboratory experiment but still, attain a poor or no grade. Examples of sterile MSA as well as plates with growth of halophili colonies. The experimental group with lid on it also showed the growth of red colonies, similar to the control. Lecture exam 2: Cell structure and bacterial growth Laboratory: Exercise 5. Results:1. Recall the history of microbiology and report the nomenclature and. Introduction. Coli bacterial cells through the transformation process (Carolina Biological Supply Co. Gram Negative Bacteria Lab Report The cell wall is composed of several layers of peptidoglycan which are held together by teichoic acids, which gives the cell wall a negative charge. 604. Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. The two forms of bacterial growth seen are with both the +pGLO plasmids because its genes connect with the LB broth, ampicillin, and arabinose. You need a reliable technique lab report your results of and biochemistry is bacillus are also requires incubation for bacterial isolates to this experiment. After all groups performed the experiment, a class data for bacterial amylase was collected. 5. The different is that one glows but it has bacteria growing over the glowing colonies and the other has an arabinose resistance that allows the glow in the colonies to be seen. Most bacteria are not this agreeable! There are two main ways to isolate organisms. Step 2: Use the sterile cooled loop and draw over the agar surface in the first section, flame and cool. See full list on sciencecompany. The growth of a bacterial population occurs in a geometric or exponential manner: with each division cycle (generation), one cell gives rise to 2 cells, then 4 cells, then 8 cells, then 16, then 32, and so forth. In addition, nutrients are added to the agar to provide a food source for the bacteria culture. temperature on microbial growth. Bacterial transformation Genetic transformation is the uptake and expression of DNA on living organisms, and genetic transformations require 3 conditions “ (1)a host in which DNA can be inserted (2) a mean of carrying the DNA into the host, and (3) a method for selecting and isolating the successfully transformed organisms” in this experiment we tested genetic transformation using Escherichia Coli (E. The width of the zone is compared to a standard table. This lab report will focus on controlling the growth of bacterial growth. Growth was estimated on a scale of 0-4 with 4 being the most growth. The Lab Report Template is what the students use to develop the lab report of this project that will be turned in. Rapid identification tests. The aim of this experiment is to follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the You will be doing a lab report to describe your experiment and its results. At the start of this semester in Microbiology we have discussed the importance of being able to identify different Bacteria. way of gene variability in bacteria. experiment includes a pH meter, waterbath, hotplate, vortex mixer, benchtop centrifuge and spectrophotometer. Table 1: Class Average for Bacterial Amylase activity. It is estimated that only 0. This was included so you can get more detail about the actual steps involved in bacterial transformation. docx) 573kB Oct17 16) Teaching Notes and Tips (NOTE to Instructors: The steps of this experiment may completed by different lab groups and the data combined or each lab group may do all steps Lab reports are an essential part of all laboratory courses and usually a significant part of your grade. Much like Griffith’s experiment, we conducted our own transformation lab using E. For example, one in perhaps a million trypanosomes may undergo a In reality, exponential growth is only part of the bacterial life cycle, and not representative of the normal pattern of growth of bacteria in Nature. T. Fall/2012. The different is that one glows but it has bacteria growing over the glowing colonies and the other has an arabinose resistance that allows the glow in the colonies to be seen. Bacteria - Bacteria - Growth of bacterial populations: Growth of bacterial cultures is defined as an increase in the number of bacteria in a population rather than in the size of individual cells. 2. When a fresh medium is inoculated with a given number of cells, and the population growth is monitored over a period of time, plotting the data will yield a typical bacterial growth curve (Figure Pure samples of Microccus luteus and Serratia marcesceus were obtained. While partaking in the bacterial growth assignment a special emphasis will be placed on control of the bacteria to ensure there is no catastrophic spread of the bacteria. marcescens is a gram negative rod shaped bacteria, containing pigment prodigiosin. Most of the bacteria grow at neutral pH (60. While the alternative hypothesis is that the E. Control:(-DNA)On plate 1 since there is no antibiotic present the wild-type bacteria grew normally and formed a "lawn" across the whole plate. An example of a Transformation Lab A Plasmid Discovery Labratory 6, AP Biology Abstract. Bacterial motility . Some bacteria can be beneficial while others can be harmful. 5. Gupta. Provide examples of why bacterial growth rates have to be measurable/ characterized Since MSA is a selective media, which selects for Gram-positive bacteria, a Gram stain was accomplished on this growth to determine if it was the Gram-positive bacteria. To learn aseptic The only plate that showed cell growth was the plate containing the mixture of the two bacterial strains. bacterial growth experiment lab report